Cell Interactions in the Differentiation of a Melanotic Tumor in Drosophila

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72644/1/j.1432-0436.1979.tb01002.x.pd

Morphogenic Effects of Halogenated Thymidine Analogues on Drosophila

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/74738/1/j.1432-0436.1975.tb00853.x.pd

Topology of the caudal fat body of the tumorw mutant of Drosophila melanogaster

Hereditary melanotic tumors in the tumorw strain of Drosophila melanogaster are known to involve encapsulation of the caudal fat body by the larval hemocytes. The encapsulated masses are subsequently melanized. The present study shows that the chain of events preceding encapsulation includes disintegration of the basement membrane of the caudal fat body and the appearance of particulate materials between and around the dissociating fat cells.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/22314/1/0000759.pd

Distribution of bromouracil in the pyrimidine oligonucleotides of drosophila DNA

DNA was isolated from Drosophila melanogaster larvae fed radioactive thymidine (TdR), bromodeoxyuridine (BUdR), or one of these nucleosides together with 5-fluorouracil (FU) under identical conditions. The four DNA samples designated T-DNA, B-DNA, T(F)-DNA, and B(F)-DNA respectively were hydrolyzed with formic acid-diphenylamine, and the pyrimidine oligonucleotides of various chain lengths (isostichs) were fractionated by DEAF-cellulose chromatography. The distributions of the labeled thymine and bromouracil (BU) residues among the pyrimidine isostichs were determined, and compared for the four DNAs. These comparisons indicated the following: 1. (1) Similarity of T-DNA and T(F)-DNA with respect to labeled thymine distribution among the pyrimidine isostichs (using the mononucleotide to pentanucleotide tracts for statistical comparisons) showed that TdR entering DNA synthesis via the salvage pathway has the same distribution whether the synthetic pathway is operating normally or is inhibited by FU treatment.2. (2) Quantitative comparison of the distribution of BU among the isostichs of B-DNA. and B(F)-DNA with the distribution of labeled thymine in T-DNA showed significant differences, indicating that the organism does not accept BUdR indiscriminately at all thymine sites for DNA synthesis.3. (3) Comparison of the distribution of BU in B-DNA and B(F)-DNA showed that isostichs 1 and 3 differ significantly. The elution profiles of the isostichs also differed qualitatively between these DNAs. This analysis confirms differential incorporation of BUdR into Drosophila DNA in the presence of a thymidylate inhibitor.The observations on BUdR incorporation in Drosophila DNA have been discussed with relation to the high frequency of somatic cell mutations induced in imaginal disc cells by treatment with the analog in the presence of inhibitors of thymidylate synthetase.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/22176/1/0000607.pd

Microtubule inhibitors block the morphological changes induced in Drosophila blood cells by a parasitoid wasp factor

The shape change of Drosophila melanogaster blood cells (lamellocytes) from discoidal to bipolar that is caused by a factor from the female parasitoid Leptopilina heterotoma is blocked by the tubulin inhibitors vinblastine and vincristine in vitro. The actin inhibitor, cytochalasin B, causes arborization of Drosophila lamellocytes and acts synergistically with the wasp factor to alter lamellocyte morphology. Lamellocyte arborization induced by cytochalasin B is blocked by simultaneous treatment with vinblastine. These observations indicate that the changes in lamellocyte shape induced by both the wasp factor and cytochalasin B require microtubule assembly.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42825/1/18_2005_Article_BF01951775.pd

Properties of the larval hemocytes of Drosophila melanogaster

Contrary to Srdić and Gloor's report, we find crystal cells (cc) in the lymph glands of D. melanogaster larvae; the size and number of inclusions in the cc cannot be used to distinguish the 2 sibling species, D. melanogaster and D. simulans ; cc in the hemocoel are not phagocytic cells; the surface properties of the lamellocytes are consistent with their derivation from plasmatocytes and not cc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42706/1/18_2005_Article_BF01976142.pd

Morphogenic effects of halogenated thymidine analogs on Drosophila III. 5-Iododeoxyuridine

Die Häufigkeit der durch 5-Iododeoxyuridin (IUdR) bei Drosophila induzierten Abnormalitäten kann durch gleichzeitige Fütterung der Larven mit 5-Fluorouracil (FU) erhöht werden. Die Menge des in die Drosophila -DNS inkorporierten IUdR ist bei Anwesenheit von FU höher; die Verteilung dieser IUdR-DNS im CsCl-Dichtegradienten ist verschieden von der in Abwesenheit von FU synthetisierten DNS.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42534/1/18_2005_Article_BF01928722.pd